Can villagers leave without letting you stop them?

I think I remember Peewee doing it once in City Folk. It's been years though so I'm not sure how accurate that is.
 
My Muffy left after I TT'd between two days, even though I talked to her, and she and I were close.
Then I loaded my game and was informed by Miserabelle, that Muffy was moving. O_o
 
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